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KMID : 0903519990420030246
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1999 Volume.42 No. 3 p.246 ~ p.251
Changes in Antioxidant and Antioxidant Enzymes Activities of Soybean Leaves Subjected to Water Stress



Abstract
This experiment was carried out to elucidate and investigate the changes in the content of antioxidants and the activities of antioxidant enzymes in the leaves of soybean subjected to water stresses. The results obtained were as follows; Leaves of soybeans subjected to water stresses have showed the differences in the activities of the antioxidant enzymes. In eunhakong, the activity of APOX was increased within a few days, but that of GR was decreased, whereas the activities of APOX and GR were gradually decreased in eunhakong. The activity of MDHAR of the leaves of eunhakong subjected to drought stress was gradually increased within 4days, whereas that of flooding was increased within 2days. We are supposed that the activities of APOX and MDHAR are coupled to maintain ascorbate concentration. In eunhakong, the relative activity of DHAR subjected to flooding was higher than that of drought. These results imply that DHAR is the only enzyme participating in the regeneration of ascorbate when the activity of MDHAR was limited by the deficiency of NADPH. The contents of ascorbate and reduced glutathione subjected to drought stress decreased continually, whereas those subjected to flooding stress recovered after five days of treatment.
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